It requires some thought and usually comes with experience, generally during postgraduate research. This knowledge cannot be picked up by following a fixed recipe for a class practical. In order to purify any protein you need to know which separation techniques are likely to be most effective under the circumstances and, probably more important, which techniques are not. Lectures and textbooks can cover the theoretical aspects of protein purification and laboratory classes can teach the practical techniques, but there are other topics which are difficult to learn by conventional methods.
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